Patricia Coltri - USP
 

In our lab, we are interested on splicing regulation in eukaryotes. Control of gene expression depends on extensive RNA processing. Most genes are composed by exons and introns. Splicing is the removal of intervening sequences, or introns, and ligation of exons into mature RNAs. These RNAs can be directed to translation or, in some cases, perform other roles in the cell. This process is performed by a complex macromolecular complex called the spliceosome. 

The spliceosome is a multi-megadalton machinery assembled on every RNA transcript. It is composed of five small nuclear RNAs (U1, U2, U4, U5 and U6 snRNAs) and over 100 different proteins. Most of these are core components of the complex whereas some are transiently associated regulatory factors.

We investigate the mechanisms that direct and guide the splicing machinery to the transcript. We use molecular and cell biology techniques to understand how this activity is modulated by different proteins and spliceosome components as well as by different sequences on transcripts.

If you are interested in joining us, please send your info to:


coltri [at] usp [dot] br

 

 

   

 

Lab members

 

 

 

 

Gisela T. Ramos, technician

 

 

Pos-doc

 
 

Dra. Rosicler Lázaro Barbosa

Identification and characterization of Trypanosoma spliceosomes isolated under different conditions
Funding: PNPD/CAPES


 
 

 

Graduate students

   
 

Marcelo Machado Paiva

Splicing regulation and spliceosome composition of miRNA-containing introns.
Funding: CAPES

 

 

 

 

Guilherme Henrique Gatti da Silva

Regulation of eukaryotic miRNA splicing
Funding: CAPES


 

 

Undergraduate students

   
 

Arthur Tributino Menezes
GFP reporter transcript construction and splicing analysis in human cells.

 
 

Helder Nagasse

Splicing efficiency in miRNA-containing transcripts


 

 

Past members

   
 

Juliana Pimentel Galhardo


 

Marcelo Machado Paiva